David Vendrami, recently recruited Early Stage Researcher to the CACHE project, talks mitochondrial DNA:
“I’ve had a pretty busy week: in addition to working on my CACHE project, I had to deal with some (tedious) bureaucratic stuff which, of course, I’m not going to talk to you about.
In the last few days, I focused on mainly three activities: first of all, training Cara, who is an undergraduate student, on the laboratory procedure to obtain mitochondrial DNA sequences and on the analysis of them using three different computer programs. This was a really pleasant activity (my first experience in supervising a student) and the both of us are really excited to see the final results.
In the meanwhile, I’ve been designing microsatellite multiplex reactions and playing around with PCR conditions to find out which parameters lead to better results. The data generated in this way will be combined with the data coming from the mitochondrial DNA sequences to study the population genetic structure of nine populations of King scallop (Pecten maximus) from Northern Ireland.
Finally, I’ve been chatting over Skype with a good friend of mine from Italy. He is going to collect for me thirty samples of Native oyster (Ostrea edulis) in the northern part of the Adriatic Sea, so we were discussing about sampling protocols and timing.”