Towards the performance of ‘in-house’ ddRAD sequencing

Recent recruit, David Vendrami, gives an insight into the technicalities of ddRAD sequencing:

“A part of my project consists of the exploitation of ‘Next Generation Sequencing’ techniques to produce a huge and highly informative amount of genomic data. Specifically, I have to produce what is known as a ‘ddRAD library’, which is a collection of carefully selected DNA fragments which are going to be sequenced to produce, as just mentioned, a huge and highly informative amount of genomic data. Currently, at the University of Bielefeld, a protocol for the production of ddRAD libraries is not set up (at least, not yet). So, I joined the Florian Leese’s Lab at the University of Bochum, where ddRAD library preparation is performed, with the double aim of learning how to produce a ddRAD library and ‘import’ the protocol to Bielefeld.

The week I spent in Bochum was really interesting and I learnt a lot from it; additionally I produced, with the help of two great PhD students (Hannah Schweyen and Martina Weiss), my first ddRAD library. The ‘recipe’ to generate this kind of library is definitely the most complex protocol I’ve ever dealt with and, for this reason, I’m looking forward to produce many more of them and become as familiar as possible with this extremely promising technique.

Then, I got back to Bielefeld and I brought my ddRAD library to the Cebitec (“Centrum für Biotechnologie”) in order to have it sequenced. I am now waiting for the results and I’m really excited to look at them. And what it makes me even more excited, is that the Cebitec is located within the University of Bielefeld and it is part of the University of Bielefeld. This means that if I’ll be able to import the protocol for the generation of ddRAD libraries in our laboratory, it will be possible to perform the whole process ‘in-house’, within the University of Bielefeld!! Crossed-fingers!”

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